4DS-Lyser Solution

Introduction 4DS-Lyser Solution

4DS Lysing Solution, developed by the innovative Labnovation company, is a micro-pure, structured biochemical reagent that controls cellular fluorescence and sample preparation in diagnostic systems.

Advanced 5-part white blood cell differential counting (WBC Differential) on modern hematology analyzers depends on laser flow cytometry and highly sensitive fluorescent dyes, rather than simple chemical reactions. 4DS Lysing Solution (the commercial and technical counterpart of Lysercell 4DS reagent), developed by the innovative Labnovation company, is a micro-pure, structured biochemical reagent that controls cellular fluorescence and sample preparation in diagnostic systems. Packaged in a specialized 3-pack (each vial containing 42 mL), this product is precisely calibrated and designed for complete biochemical compatibility with the cell-tracking and size-measurement channels in advanced Sysmex XN-Series autoanalyzers (such as XN-350, XN-550, XN-1000, and higher).

Strategic Applications and Flow Cytometry Mechanism of Sysmex 4DS Lyse

Equipped with permeabilizing agents, this advanced biochemical reagent performs the following key roles in the WDF (white blood cell differential) measurement channel of Sysmex instruments:

• Enriched Membrane Permeabilization: Uses a calibrated surfactant formulation to create micron-scale pores in blood leukocyte membranes without disrupting nuclear and cytoplasmic structures.

• Facilitating Fluorescent Dye Ingress (Fluorocell Staining Prep): Prepares the cellular environment to enable rapid, uniform entry of fluorescent dyes (such as Fluorocell WDF) into the cell, where they bind nucleic acids (DNA/RNA) for optical differentiation.

• Advanced White Blood Cell Differentiation (WBC Differential): Creates an optimal matrix for precise sorting and categorization of lymphocytes, monocytes, neutrophils, and eosinophils, and detects immature cells based on laser scatter intensity.

• Complete Red Blood Cell Lysis and Digestion (RBC Lysis): Rapidly clears and digests erythrocyte membranes to prevent physical noise in the optical signal pathways of flow cytometry sensors.

Technical Specifications and Device Compatibility

Quality Features and Operational Benefits of 42 mL Vials

• Micro-Kit Configuration and High Stability: Packaging the product in 3-pack sets of 42 mL vials prevents active lytic compounds from degrading due to prolonged exposure in large containers, thereby increasing shelf life.

• Nano-Filtered Biochemical Purity: Processed on fully automated production lines with precision filtration to remove unwanted micro-particles, reducing background counts to near zero.

• Full Compatibility with Delicate Sysmex Injection Needles: Viscosity and surface tension are calibrated to minimize hydraulic pressure on high-value XN-series micro-pumps.

• Fully Ready-to-Use Structure: Placed directly into the reaction chamber without dilution, eliminating human error in reagent-volume calibration.

Technical Limitations, Maintenance Tips, and Operational Interactions

To prevent optical plot errors and avoid false pathological reports, the following requirements must be observed:

• Strict Ambient Temperature Chain Adherence: Store the reagent strictly between 2 and 30°C, away from heat and direct light. Freezing disrupts active surfactant bonds, leading to incomplete hemolysis or non-uniform staining.

• Preventing Degradation and Surface Evaporation: Manage usage according to standard instrument workload schedules after opening each 42 mL vial; leaving vials open alters the concentrations of membrane-permeabilizing compounds.

• Hygiene Requirements for Suction Nozzles: Avoid touching instrument probes or needles with bare hands or unsterile wipes when placing vials into the housing to prevent cross-contamination.

Laboratory Staff FAQ

1. What role does 4DS lysing solution play in generating WDF scattergrams (WBC differential plot)?
   In the Sysmex XN-series, cells are permeabilized with the 4DS solution and tagged with fluorescent dyes before passing through the laser. The instrument then distinguishes lymphocytes, neutrophils, monocytes, and eosinophils based on cell size (Forward Scatter) and fluorescence intensity (Side Fluorescence), ensuring clear separation without overlap, and displays them on the scattergram.

2. What errors occur on Sysmex instruments when using expired or damaged lysing solution?
   Chemical degradation of this reagent causes non-uniform cell permeabilization and incomplete RBC lysis. This biochemical challenge results in cluster separation errors (WBC Scattergram Abnormal) and interferes with leukocyte counts.

3. Does this solution formulation contain toxic cyanide-based compounds?
   No; Labnovation uses modern, biocompatible, cyanide-free formulations to design this advanced reagent, enhancing laboratory staff safety and minimizing waste disposal challenges.

Price Inquiry and Purchasing Labnovation 4DS Lysing Solution

To ensure consistent and advanced flow cytometry testing on Sysmex hematology systems, it is essential to use approved, homogeneous reagents. You can purchase 4DS Lysing Solution in 3-pack sets and get expert advice on hematology kits by ordering through the Padina Vista website or reaching out to our sales team. This product comes with quality test certifications, valid expiration dates, and guarantees of authentic chemical structure.

Technical Specifications محلول لایزر 4DS

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